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KMID : 0363320020230020202
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Journal of Korean Oriental Internal Medicine
2002 Volume.23 No. 2 p.202 ~ p.211
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The Molecular Biological Study on Anti-Cancer Effects of Sagunjatang plus Cremastrae Appenediculatae Tuber on Human Stomach Cancer Cells
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Kim Jin-Seok
Ryu Bong-Ha
Ryu Ki-Won
Yoon Sang-Hyub
Kim Jin-Seong
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Abstract
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1. Background
The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism.
2. Objective
The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS).
3. Methods
After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell. MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis.
4. Result and Conclusion
Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity. decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.
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KEYWORD
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Sagunjatang, Cremastrae Appenediculatae Tuber, Human stomach cancer cells(AGS), MTT assay, Apoptosis, RT-PCR
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